PuSH - Publikationsserver des Helmholtz Zentrums München

Gemünd, I. ; Bonaguro, L.* ; Becker, M.* ; Müller, S.* ; Joos, C. ; De Domenico, E.* ; Aschenbrenner, A.C.* ; Schultze, J.L.* ; Moosmann, A. ; Beyer, M.D.*

Characterization of human CMV-specific CD8+ T cells using multi-layer single-cell omics.

Cell Rep. Methods, DOI: 10.1016/j.crmeth.2025.101085:101085 (2025)
Verlagsversion Forschungsdaten DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
In this study, we established a comprehensive workflow to collect multi-omics single-cell data using a commercially available micro-well-based platform. This included whole transcriptome, cell surface markers (targeted sequencing-based cell surface proteomics), T cell specificities, adaptive immune receptor repertoire (AIRR) profiles, and sample multiplexing. With this technique, we identified paired T cell receptor sequences for three prominent human CMV epitopes. In addition, we assessed the ability of dCODE dextramers to detect antigen-specific T cells at low frequencies by estimating sensitivities and specificities when used as reagents for single-cell multi-omics.
Altmetric
Weitere Metriken?
[➜Einloggen]
Zusatzinfos bearbeiten [➜Einloggen]
Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Bd Rhapsody ; Cd8(+) T Cells ; Cmv ; Cp: Immunology ; Tcr Sequencing ; Antigen-specific T Cells ; Dcode Dextramers ; Single-cell Multi-omics ; Systems Immunology
ISSN (print) / ISBN 2667-2375
e-ISSN 2667-2375
Zeitschrift Cell Reports Methods
Quellenangaben Band: , Heft: , Seiten: , Artikelnummer: 101085 Supplement: ,
Verlag Elsevier
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Virology (VIRO)