PuSH - Publikationsserver des Helmholtz Zentrums München

Waas, D.* ; Juraschitz, M.* ; Chen, Y.A.* ; Waltenberger, H.* ; Hammerschmidt, W. ; Zeidler, R. ; Molinaro, S.* ; Gärtner, K.*

Development of broadly applicable reference EVs expressing horseradish peroxidase.

J. Extra. Vesicles 14, 13:e70115 (2025)
Verlagsversion Forschungsdaten DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
Extracellular vesicles (EVs) hold great promise as circulating biomarkers for diagnostic and therapeutic approaches. Thus, many research groups world-wide investigate important aspects of EVs including their biology and medical significance. For this, a large number of procedures and protocols has been established making it difficult to almost impossible to compare and replicate results. Consequently, diagnostic tests remain problematic to interpret, mainly because the use of reliable reference EVs as a qualified standard has not yet gained widespread acceptance. Beyond doubt, such reference EVs are key to assess EV preparations quantitatively and to establish robust quality control processes to ensure overall quality and validity of data. To further advance the establishment of such controls, we designed and generated a new class of reference EVs expressing horseradish peroxidase (HRP) to facilitate simple and reliable EV tracing during isolation and standardization of EV purification and downstream analysis processes. HRP+ EVs can be quantified easily and with unmatched sensitivity either directly via measuring HRP activity or indirectly via immunodetection of HRP on the EV surface. We demonstrate that HRP+ EVs allow the reliable quantification of absolute EV numbers in biological or medical samples to normalize clinical specimens in liquid biopsies.
Altmetric
Weitere Metriken?
[➜Einloggen]
Zusatzinfos bearbeiten [➜Einloggen]
Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Extracellular Vesicles ; Horseradish Peroxidase ; Normalization ; Quality Control ; Standardization; Extracellular Vesicles; Biomarkers; Exosomes; Targets; View
ISSN (print) / ISBN 2001-3078
e-ISSN 2001-3078
Zeitschrift Journal of extracellular vesicles
Quellenangaben Band: 14, Heft: 8, Seiten: 13, Artikelnummer: e70115 Supplement: ,
Verlag Taylor & Francis
Verlagsort [S.l.]
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Research Unit Gene Vector (AGV)
Institute of Structural Biology (STB)