PuSH - Publikationsserver des Helmholtz Zentrums München

Borges, A. ; Miranda-Rodríguez, J.R. ; Ceccarelli, A.S.* ; Ventura, G.* ; Sedzinski, J.* ; López-Schier, H. ; Chara, O.*

ForSys: Non-invasive stress inference from time-lapse microscopy.

iScience 28:113685 (2025)
Verlagsversion Forschungsdaten DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
During tissue development and regeneration, cells interpret and exert mechanical forces that are challenging to measure in vivo. Stress inference algorithms have thus emerged as powerful tools to estimate tissue stresses. Yet, effectively incorporating tissue dynamics into these algorithms remains elusive. Here, we introduce ForSys, a Python-based software that infers intercellular stresses and intracellular pressures from time-lapse microscopy. After validation, we applied ForSys to the migrating zebrafish lateral-line primordium, revealing increased stress during the cell rounding that precedes mitosis and accurately predicting the onset of epithelial rosettogenesis. We further used ForSys to study neuromast development and uncovered mechanical asymmetries linked to cell type-specific adhesion. The software performs both static and dynamic stress inference, supports command-line use, scripting, and a user-friendly graphical interface within Fiji, and accepts segmentation inputs from EPySeg and Cellpose. This versatility of ForSys enables the analysis of spatiotemporal patterns of mechanical forces during tissue morphogenesis in vivo.
Altmetric
Weitere Metriken?
[➜Einloggen]
Zusatzinfos bearbeiten [➜Einloggen]
Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Optical Imaging; Tissue Migration; Cell Contacts; Reveals; Proliferation; Segmentation; Mechanics; Junctions; Tension; Forces; Shape
ISSN (print) / ISBN 2589-0042
e-ISSN 2589-0042
Zeitschrift iScience
Quellenangaben Band: 28, Heft: 11, Seiten: , Artikelnummer: 113685 Supplement: ,
Verlag Elsevier
Verlagsort Amsterdam ; Bosten ; London ; New York ; Oxford ; Paris ; Philadelphia ; San Diego ; St. Louis
Begutachtungsstatus Peer reviewed
Institut(e) Research Unit Sensory Biology and Organogenesis (SBO)
Förderungen BBSRC
Fondo para la Investigacio
CONICET
Novo Nor-disk Foundation Center for Stem Cell Medicine
European Research Council
LEO Foundation
Novo Nordisk Foundation
Biotechnology and Biological Sciences Research Council (BBSRC) grant
CONICET, Argentina