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Meyer, A.* ; Heidemann, M. ; Lidschreiber, M.* ; Schreieck, A.* ; Sun, M.* ; Hintermair, C. ; Kremmer, E. ; Eick, D. ; Cramer, P.*

CTD tyrosine phosphorylation impairs termination factor recruitment to RNA polymerase II.

Science 336, 1723-1725 (2012)
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In different phases of the transcription cycle, RNA polymerase (Pol) II recruits various factors via its C-terminal domain (CTD), which consists of conserved heptapeptide repeats with the sequence Tyr(1)-Ser(2)-Pro(3)-Thr(4)-Ser(5)-Pro(6)-Ser(7). We show that the CTD of transcribing yeast Pol II is phosphorylated at Tyr(1), in addition to Ser(2), Thr(4), Ser(5), and Ser(7). Tyr(1) phosphorylation stimulates binding of elongation factor Spt6 and impairs recruitment of termination factors Nrd1, Pcf11, and Rtt103. Tyr(1) phosphorylation levels rise downstream of the transcription start site and decrease before the polyadenylation site, largely excluding termination factors from gene bodies. These results show that CTD modifications trigger and block factor recruitment and lead to an extended CTD code that explains transcription cycle coordination on the basis of differential phosphorylation of Tyr(1), Ser(2), and Ser(5).
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter ELONGATION-FACTOR SPT6; TANDEM SH2 DOMAIN; TRANSCRIPTION TERMINATION; S. CEREVISIAE; CODE; COMPLEX; PROTEIN; IICTD; ASSOCIATION; SERINE-7
Sprache englisch
Veröffentlichungsjahr 2012
HGF-Berichtsjahr 2012
ISSN (print) / ISBN 0036-8075
e-ISSN 1095-9203
Zeitschrift Science
Quellenangaben Band: 336, Heft: 6089, Seiten: 1723-1725 Artikelnummer: , Supplement: ,
Verlag American Association for the Advancement of Science (AAAS)
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Functional Epigenetics (IFE)
CF Monoclonal Antibodies (CF-MAB)
Institute of Molecular Immunology (IMI)
Institute of Clinical Molecular Biology and Tumor Genetics (K.MOLBI)
POF Topic(s) 30203 - Molecular Targets and Therapies
30201 - Metabolic Health
30504 - Mechanisms of Genetic and Environmental Influences on Health and Disease
Forschungsfeld(er) Helmholtz Diabetes Center
Immune Response and Infection
PSP-Element(e) G-502890-001
G-502210-001
G-501760-001
G-501793-001
G-501490-001
DOI 10.1126/science.1219651
WOS ID WOS:000305794500055
Scopus ID 84862977456
PubMed ID 22745433
Erfassungsdatum 2012-07-12
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