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Steegmüller, T.* ; Walch, S. ; Gschwendtner, S. ; Klingl, A.* ; French, L.E.* ; Flaig, M.* ; Clanner-Engelshofen, B.M.*

Third generation genome sequencing of the endobacterium Corynebacterium kroppenstedtii subsp. demodicis reveals details of its microbe-host-Interaction with the most complex human commensal, Demodex folliculorum.

Environ. Microbiol. Rep. 18:e70374 (2026)
Verlagsversion Forschungsdaten DOI PMC
Open Access Hybrid
Creative Commons Lizenzvertrag
Demodex mites inhabit the pilosebaceous unit despite harsh environmental conditions including UV radiation, variable salinity, and cosmetics. Their recently characterized endobacterium may contribute to this resilience. This study aimed to elucidate mechanisms of the microbe-host interaction that help mites withstand environmental stress. The genome of Corynebacterium kroppenstedtii subsp. demodicis was sequenced using PacBio technology and annotated via MicroScope. Metabolic and symbiotic traits were analyzed using KEGG and compared with the Demodex folliculorum secretome from published transcriptome data. The complete 2,456,075 bp genome contains 2034 coding sequences and exhibits reduced variable genes compared to other Corynebacterium species. Primary metabolism comprises an almost complete minimal gene set but lacks two tRNA synthetases and genes for phosphatidylethanolamine and NAD+ biosynthesis. Carbohydrate pathways are incomplete and fatty acid synthase I is absent. Secondary metabolism includes complete mevalonate and β-carotene biosynthetic pathways, while the methylerythritol phosphate pathway is missing. UV protection and oxidative stress tolerance are supported by β-carotene, ClpB, RecN, MsrA, KatA, SodA, and manganese transporter SitB. The secretome contains hydrolases likely aiding mite digestion. These findings provide genomic insights into mite-bacterium symbiosis and follicular adaptation. All functional inferences are based on genomic data and in silico predictions; experimental validation remains to be established.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Demodicidae ; Endosymbiont ; Mite ; Parasite ; Symbiosis; Database; Kegg
ISSN (print) / ISBN 1758-2229
e-ISSN 1758-2229
Zeitschrift Environmental Microbiology Reports
Quellenangaben Band: 18, Heft: 3, Seiten: , Artikelnummer: e70374 Supplement: ,
Verlag Wiley
Verlagsort 111 River St, Hoboken 07030-5774, Nj Usa
Begutachtungsstatus Peer reviewed
Institut(e) Research Unit Comparative Microbiome Analysis (COMI)
Förderungen Medical & Clinician Scientist Program (MCSP) at LMU Munich