PuSH - Publikationsserver des Helmholtz Zentrums München

Chadha, Y. ; Kukhtevich, I. ; Stegmaier, T. ; Padovani, F. ; Schneider, R. ; Schmoller, K.M.

Single-cell imaging reveals a key role of Bck2 in yeast cell size adaptation to nutrient challenges.

J. Cell Biol. 225:e202410020 (2026)
Verlagsversion Forschungsdaten DOI
Open Access Hybrid
Creative Commons Lizenzvertrag
Cell size is tightly controlled to optimize cell function. In Saccharomyces cerevisiae, multiple regulators of cell size in steady-state conditions have been identified, such as the G1/S activators Cln3 and Bck2 and the inhibitor Whi5. Individual deletions of these regulators alter the mean cell volumes. However, size homeostasis remains largely intact. Here, we show that although the roles of Bck2 and Cln3 for cell size regulation appear largely redundant in steady state, a switch from fermentable to nonfermentable growth media reveals a unique role for Bck2 in cell size adaptation. We use live-cell microscopy and machine learning-assisted image analysis to track single cells and their progeny through the nutrient switch. We find that after the switch, bck2Δ cells experience longer cell cycle arrests and more arrest-associated enlargement than wild-type, whi5Δ, or cln3Δ cells, indicating that Bck2 becomes the critical G1/S activator in changing nutrients. Our work demonstrates that studying size regulation during nutrient shifts can resolve apparent redundancies observed in steady state.
Altmetric
Weitere Metriken?
[➜Einloggen]
Zusatzinfos bearbeiten [➜Einloggen]
Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
ISSN (print) / ISBN 0021-9525
e-ISSN 1540-8140
Zeitschrift Journal of Cell Biology, The
Quellenangaben Band: 225, Heft: 12, Seiten: , Artikelnummer: e202410020 Supplement: ,
Verlag Rockefeller University Press
Begutachtungsstatus Peer reviewed
Institut(e) Institiute of Functional Epigenetics (IFE)