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Sharma, S. ; Radl, V. ; Hai, B. ; Kloos, K. ; Mrkonjic, M. ; Engel, M. ; Schauss, K. ; Schloter, M.

Quantification of functional genes from procaryotes in soil by PCR.

J. Microbiol. Methods 68, 445-452 (2007)
DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
Controlling turnover processes and fluxes in soils and other environments requires information about the gene pool and possibilities for its in situ induction. Therefore in the recent years there has been a growing interest in genes and transcripts coding for metabolic enzymes. Besides questions addressing redundancy and diversity, more and more attention is given on the abundance of specific DNA and mRNA in the different habitats. This review will describe several PCR techniques that are suitable for quantification of functional genes and transcripts such as MPN-PCR, competitive PCR and real-time PCR. The advantages and disadvantages of the mentioned methods are discussed. In addition, the problems of quantitative extraction of nucleic acid and substances that inhibit polymerase are described. Finally, some examples from recent papers are given to demonstrate the applicability and usefulness of the different approaches.
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Publication type Article: Journal article
Document type Scientific Article
Keywords quantitative PCR; real-time PCR; MPN-PCR; competitive PCR; microbial activity
ISSN (print) / ISBN 0167-7012
e-ISSN 1872-8359
Journal Journal of Microbiological Methods
Quellenangaben Volume: 68, Issue: 3, Pages: 445-452 Article Number: , Supplement: ,
Publisher Elsevier
Publishing Place Amsterdam
Reviewing status Peer reviewed
Institute(s) Institute of Soil Ecology (IBOE)