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Open Access Green as soon as Postprint is submitted to ZB.
Study on the multiple sites binding of human serum albumin and porphyrin by affinity capillary electrophoresis.
J. Chromatogr. B 768, 211-214 (2002)
Equations to describe the two sites binding between proteins and ligands were deduced. According to these equations, not only the binding constants, but also the mole fraction of proteins in different forms could be obtained. Using the published data on the interaction between human serum albumin (HSA) and three kinds of porphyrin (coproporphyrin (CP), uroporphyrin I (UP) and protoporphyrin (PP)), a further study on their binding was carried out. It was concluded that there may exist two binding sites with the binding constants at the first site. proved to be the preferential one, being 6.50 x 10(5) 1.94 x 10(6) and 8.94 x 10(5). respectively. In addition. it was also demonstrated that the two binding sites of HSA with CP and UP might be of different kinds, though those of HSA and PP were of the same kind but at different positions.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Affinity capillary electrophoresis; Protein binding; Human serum albumin; Porphyrin
ISSN (print) / ISBN
1570-0232
e-ISSN
1873-376X
Journal
Journal of Chromatography
Quellenangaben
Volume: 768,
Pages: 211-214
Publisher
Elsevier
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institute of Ecological Chemistry (IOEC)