PuSH - Publication Server of Helmholtz Zentrum München: Quantitative and qualitative precision improvements by effective mobility-scale data transformation in capillary electrophoresis analysis.

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Schmitt-Kopplin, P. ; Garmash, A.V.* ; Kudryavtsev, A.V.* ; Menzinger, F. ; Perminova, I.V.* ; Hertkorn, N. ; Freitag, D. ; Petrosyan, V.S.* ; Kettrup, A.

Quantitative and qualitative precision improvements by effective mobility-scale data transformation in capillary electrophoresis analysis.

Electrophoresis 22, 77-87 (2001)

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Open Access Green as soon as Postprint is submitted to ZB.

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By transforming the time-based x-axis of electropherograms in capillary zone electrophoresis (CZE) into the corresponding effective mobility-scale, we propose a simple and robust data representation for a better qualitative and quantitative capillary electrophoresis (CE) analysis. The time scale of the raw electrophoretic data (detection signal versus time) is transformed into an effective electrophoretic mobility scale (mu eff-scale) with account of the electroosmotic flow (EOF) peak or of an internal standard of known effective mobility. With the new scaling (detection signals versus effective mobility), the obtained electropherograms are more representative of the velocity-based electrophoretic separation and the comparison of complete electropherograms is directly possible. This is of importance when tracking peaks in real samples where alteration in EOF stability can occur or when comparing electrophoretic runs from different experimental setups (independence in column length and voltage). Beside the qualitative possibilities, a quantitative improvement is achieved in the mu eff-scale with significant better peak area reproducibility and equal to more precision in quantitative analysis than with the primary time-scale integration.

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