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Frommberger, M. ; Schmitt-Kopplin, P. ; Menzinger, F. ; Albrecht, V. ; Schmid, M. ; Eberl, L.* ; Hartmann, A. ; Kettrup, A.

Analysis of N-acyl-l-homoserine lactones produced by Burkholderia cepacia with partial filling micellar electrokinetic chromatography - electrospray ionization-ion trap mass spectrometry.

Electrophoresis 24, 3067-3074 (2003)
DOI
Open Access Green as soon as Postprint is submitted to ZB.
A method for the analysis of N-acyl-L-homoserine lactones (AHLs) with micellar electrokinetic chromatography coupled to electrospray ionization-ion trap mass spectrometry, combining the flexibility of capillary electrophoresis with the unmatched structural information provided by mass spectrometry is presented. Different surfactants were evaluated, with sodium dodecyl sulfate (SDS) yielding the best results considering sensitivity and flexibility. We examined the interaction of AHLs with the SDS micelles at different analysis conditions and applied the optimized method to the analysis of a real bacterial sample. Two AHLs from Burkholderia cepacia colonizing the rhizosphere of traditional Indian rice cultivars could be unambiguously determined in an ethyl acetate extract with high resolution flexibility.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Burkholderia cepacia; Electrospray ionization-mass specrometry; Micellar electrokinetic chromatography; N-Acyl-l-homoserine; Quorum sensing
ISSN (print) / ISBN 0173-0835
e-ISSN 1522-2683
Journal Electrophoresis
Quellenangaben Volume: 24, Issue: 17, Pages: 3067-3074 Article Number: , Supplement: ,
Publisher Wiley
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Ecological Chemistry (IOEC)
Institute of Soil Ecology (IBOE)