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Schmitt-Kopplin, P. ; Kettrup, A.

Capillary electrophoresis - electrospray ionization - mass spectrometry for the characterization of natural organic matter : An evaluation with free flow electrophoresis-off-line flow injection electrospray ionization-mass spectrometry.

Electrophoresis 24, 3057-3066 (2003)
DOI
Open Access Green as soon as Postprint is submitted to ZB.
The separation of Suwannee River natural organic matter (NOM) with capillary zone electrophoresis hyphenated to electrospray ionization-mass spectrometry (CZE-ESI-MS) is presented. The obtained electropherograms and signal distributions are comparable to the mobility distributions obtained with more classical UV detection. A direct comparison of the results was possible with free-flow electrophoresis (FFE), which allows an upscaling of the CZE method and the analysis of the collected fractions in an off-line modus with flow-injection electrospray ionization-mass spectrometry (FI-ESI-MS). The changes of the m/z distributions with mobility are very similar with both methods and show a decrease of the m/z with increasing electrophoretic mobility in the humic hump at alkaline pH; superimposed on this hump a low-molecular-weight fraction migrates at lower mobility. The analysis of benzene carboxylic acids, glycerrhycic acid as well as oligomers of polystyrene sulfonic acid and polyacrylic acid additionally illustrates possible fragmentation, formation of adducts and multiplicity of the charges of the molecules prior to MS detection. These hardly controllable difficulties add a challenge to the interpretation of the obtained m/z distributions of NOM in terms of charge and mass distributions of molecules present in the NOM mixture.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Capillary electrophoresis; Capillary zone electrophoresis; Electrospray ionization; Free-flow electrophoresis; Humic substances; Mass spectrometry; Natural organic matter
ISSN (print) / ISBN 0173-0835
e-ISSN 1522-2683
Journal Electrophoresis
Quellenangaben Volume: 24, Issue: 17, Pages: 3057-3066 Article Number: , Supplement: ,
Publisher Wiley
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Ecological Chemistry (IOEC)