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Characteristics of electroosmotic flow and migration of neutral solutes under stepwise gradient elution of capillary electrochromatography.
Electrophoresis 23, 2417-2423 (2002)
A theoretical study on the velocity of electroosmotic flow (EOF) and the retention times of neutral solutes under multiple-step gradient of capillary electrochromatography (CEC) was carried out, focusing on that with three kinds of mobile phases. Through the model computations, the detaining time of the second kind of mobile phase in the column was proved to play an important role in affecting EOF. The variation speed of EOF was shown to be determined by the differences among dead times in different steps. In addition, the prediction of the retention times of 13 aromatic compounds under gradient mode was performed with the deduced equations. A relative error below 3.3% between the calculated and experimental values was obtained, which demonstrated the rationality of the theoretical deduction. Our study could not only improve the comprehension of stepwise gradient elution, but also be of significance for the further optimization of separation conditions in the analysis of complex samples.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Capillary electrochromatography; Electroosmotic flow; Retention of neutral solutes; Stepwise gradient elution
ISSN (print) / ISBN
0173-0835
e-ISSN
1522-2683
Journal
Electrophoresis
Quellenangaben
Volume: 23,
Issue: 15,
Pages: 2417-2423
Publisher
Wiley
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institute of Ecological Chemistry (IOEC)