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Open Access Gold as soon as Publ. Version/Full Text is submitted to ZB.
Direct visualization of cell division using high-resolution imaging of M-phase of the cell cycle.
Nat. Commun. 3:1076 (2012)
Current approaches to monitor and quantify cell division in live cells, and reliably distinguish between acytokinesis and endoreduplication, are limited and complicate determination of stem cell pool identities. Here we overcome these limitations by generating an in vivo reporter system using the scaffolding protein anillin fused to enhanced green fluorescent protein, to provide high spatiotemporal resolution of mitotic phase. This approach visualizes cytokinesis and midbody formation as hallmarks of expansion of stem and somatic cells, and enables distinction from cell cycle variations. High-resolution microscopy in embryonic heart and brain tissues of enhanced green fluorescent protein-anillin transgenic mice allows live monitoring of cell division and quantitation of cell cycle kinetics. Analysis of cell division in hearts post injury shows that border zone cardiomyocytes in the infarct respond with increasing ploidy, but not cell division. Thus, the enhanced green fluorescent protein-anillin system enables monitoring and measurement of cell division in vivo and markedly simplifies in vitro analysis in fixed cells.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
CARDIOMYOCYTE DNA-SYNTHESIS; STEM-CELLS; MYOCARDIAL-INFARCTION; IN-VIVO; MAMMALIAN CARDIOMYOCYTES; HUMAN HEART; MOUSE; NUCLEI; PROLIFERATION; PROGENITORS
ISSN (print) / ISBN
2041-1723
e-ISSN
2041-1723
Journal
Nature Communications
Quellenangaben
Volume: 3,
Article Number: 1076
Publisher
Nature Publishing Group
Publishing Place
London
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institute of Stem Cell Research (ISF)