PuSH - Publication Server of Helmholtz Zentrum München

Ngigi, A.N.* ; Getenga, Z.M.* ; Dörfler, U. ; Boga, H.I.* ; Kuria, B.* ; Ndalut, P.* ; Schroll, R.

Effects of carbon amendment on in situ atrazine degradation and total microbial biomass.

J. Environ. Sci. Health B 48, 40-48 (2013)
DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
This study elucidates the effects of carbon amendment on metabolic degradation of atrazine (6-chloro-N(2)-ethyl-N(4)-isopropyl-1,3,5-triazine-2,4-diamine) and total microbial biomass in soil. Degradation of (14)C-ring-labelled atrazine was monitored in laboratory incubations of soils supplemented with 0, 10, 100 and 1000 μg g(-1) sucrose concentrations. An experiment to determine the effect of carbon amendment on total microbial biomass and soil respiration was carried out with different concentrations of sucrose and non-labelled atrazine. The soils were incubated at a constant temperature and constant soil moisture at water potential of -15 kPa and a soil density of 1.3 g cm(-3). Mineralization of (14)C-ring-labelled atrazine was monitored continuously over a period of 59 d in the first experiment. The CO(2) production was monitored for 62 d in the second experiment and microbial biomass determined at the end of the incubation period. The addition of 1000 μg g(-1) sucrose reduced atrazine mineralization to 43.5% compared to 51.7% of the applied amount for the treatment without sucrose. The addition of 1000 μg g(-1) sucrose modified the transformation products to 1.08 μg g(-1) deisopropylatrazine (DIA), 0.32 μg g(-1) desethylatrazine (DEA) and 0.18 μg g(-1) deisopropyl-2-hydroxyatrazine (OH-DIA). Treatment without sucrose resulted in formation of 0.64 μg g(-1) hydroxyatrazine (HA), 0.28 μg g(-1) DIA and 0.20 μg g(-1) OH-DIA. Atrazine dealkylation was enhanced in treatments with 100 and 1000 μg g(-1) of sucrose added. HA metabolite was formed in the control (no sucrose) and in the presence of 10 μg g(-1) of sucrose, whereas DEA was only detected in treatment with 1000 μg g(-1) sucrose. Results indicate that total microbial biomass increased significantly (P < 0.001) with the addition of 1000 μg g(-1) sucrose.
Altmetric
Additional Metrics?
[➜Log in]
Edit extra informations Login
Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Atrazine, carbon amendment, mineralization, metabolites, microbial biomass; ACCELERATED DEGRADATION; HERBICIDE ATRAZINE; ORGANIC AMENDMENTS; CYANURIC ACID; SOIL; MINERALIZATION; BIODEGRADATION; NITROGEN; PSEUDOMONAS; STRAIN
ISSN (print) / ISBN 0360-1234
e-ISSN 1532-4109
Journal Journal of Environmental Science and Health, Part B
Quellenangaben Volume: 48, Issue: 1, Pages: 40-48 Article Number: , Supplement: ,
Publisher Dekker
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Soil Ecology (IBOE)