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Open Access Green as soon as Postprint is submitted to ZB.
Aberrant neurogenesis after stroke: A retroviral cell labeling study.
Stroke 43, 2468-2475 (2012)
Background and Purpose-Adult neurogenesis in the dentate gyrus is a unique form of brain plasticity that is strongly stimulated after stroke. We investigate the morphological properties of new granule cells, which are born and develop after the ischemic insult, and query whether these adult-born neurons properly integrate into the pre-existing hippocampal circuitries. Methods-Two well-established models were used to induce either small cortical infarcts (photothrombosis model) or large territorial infarcts (transient middle cerebral artery occlusion model). New granule cells were labeled 4 days after the initial insult by intrahippocampal injection of a retroviral vector encoding green fluorescent protein and newborn neurons were morphologically analyzed using a semiautomatic Neurolucida system and confocal laser scanning microscopy at 6 weeks. Results-Approximately 5% to 10% of newborn granule cells displayed significant morphological abnormalities comprising additional basal dendrites and, after middle cerebral artery occlusion, also ectopic cell position. The extent of morphological abnormalities was higher after large territorial infarcts and seems to depend on the severity of ischemic damage. An increased portion of mushroom spines in aberrant neurons suggests stable synaptic integration. However, poststroke generated granule cells with regular appearance also demonstrate alterations in dendritic complexity and spine morphology. Conclusions-The remarkable stimulation of dentate neurogenesis after stroke coincides with an increased rate of aberrantly integrated neurons, which may contribute to functional impairments and, hypothetically, favor pathogenesis of adjustment disorders, cognitive deficits, or epilepsy often seen in stroke patients. (Stroke. 2012;43:2468-2475.)
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Adult Neurogenesis ; Dentate Gyrus ; Photothrombosis ; Plasticity ; Retroviral Vectors; DENTATE GRANULE CELLS; NEWLY GENERATED NEURONS; ADULT HIPPOCAMPUS; STATUS EPILEPTICUS; CORTICAL INFARCTS; SYNAPSE FORMATION; BASAL DENDRITES; BRAIN; BORN; GYRUS
ISSN (print) / ISBN
0039-2499
e-ISSN
1524-4628
Journal
Stroke
Quellenangaben
Volume: 43,
Issue: 9,
Pages: 2468-2475
Publisher
Lippincott Williams & Wilkins
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institute of Developmental Genetics (IDG)