Home
Deutsch
Advanced Search
Browse by ...
Publication overview
Contact persons
Help
DOI
PMC
 |
|
Open Access Green as soon as Postprint is submitted to ZB.
Molecular live cell bioimaging in stem cell research.
Ann. NY Acad. Sci. 1266, 18-27 (2012)
Functional heterogeneity within stem and progenitor cells has been shown to influence cell fate decisions. Similarly, intracellular signaling activated by external stimuli is highly heterogeneous and its spatiotemporal activity is linked to future cell behavior. To quantify these heterogeneous states and link them to future cell fates, it is important to observe cell populations continuously with single cell resolution. Live cell imaging in combination with fluorescent biosensors for signaling activity serves as a powerful tool to study cellular and molecular heterogeneity and the long-term biological effects of signaling. Here, we describe these methodologies, their advantages over classical approaches, and we illustrate how they could be applied to improve our understanding of the importance of heterogeneous cellular and molecular responses to external signaling cues.
Altmetric
Additional Metrics?
Edit extra informations
Login
Publication type
Article: Journal article
Document type
Scientific Article
Keywords
biosensors; cytokines; M-CSF; signaling; lineage choice; stem cell; imaging; time lapse; Protein-Kinase-C; Stimulating Factor-I; Hematopoietic Progenitor Cells; Infrared Fluorescent Proteins; Receptor Tyrosine Kinase; M-Csf Receptor; Self-Renewal; Lineage Commitment; Living Cells; Scaffolding Protein
ISSN (print) / ISBN
0077-8923
e-ISSN
1749-6632
Quellenangaben
Volume: 1266,
Issue: 1,
Pages: 18-27
Publisher
New York Academy of Sciences
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Research Unit Stem Cell Dynamics (SCD)