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Baldini, F.* ; Bolzoni, L.* ; Giannetti, A.* ; Keß, M. ; Krämer, P. ; Kremmer, E. ; Porro, G.* ; Senesi, F.* ; Trono, C.*

A new procalcitonin optical immunosensor for POCT applications.

Anal. Bioanal. Chem. 393, 1183-1190 (2009)
DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
A new immunosensor for the determination of procalcitonin was developed. A sandwich assay format was implemented on a polymethylmetacrylate optical biochip, opportunely shaped in order to obtain several flow channels and potentially suitable for point of care testing applications. The sandwich format makes use of two new rat monoclonal antibodies. The capture antibody was covalently immobilised on the surface of the plastic chip, and the detection antibody was labelled with DY647 dye. Different combinations of capture and detection antibodies were investigated, and particular attention was devoted in order to avoid the nonspecific adsorption. A limit of detection of 0.088 mg L-1 was achieved within the working range of 0.28-50 mg L-1 in buffer samples. The assay was also implemented in human serum, and 0.2 and 0.7-25 mg L-1 were the attained limit of detection and working range, respectively.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Optical biochip; Immunosensor; Procalcitonin; PMMA; Fluorescence anisotropy; POCT; serum procalcitonin; infection; bacterial; sepsis
Language
Publication Year 2009
HGF-reported in Year 2009
ISSN (print) / ISBN 1618-2642
e-ISSN 1618-2650
Journal Analytical and Bioanalytical Chemistry
Quellenangaben Volume: 393, Issue: 4, Pages: 1183-1190 Article Number: , Supplement: ,
Publisher Springer
Publishing Place Heidelberg
Reviewing status Peer reviewed
Institute(s) Institute of Ecological Chemistry (IOEC)
Institute of Molecular Immunology (IMI)
Research Unit Microbe-Plant Interactions (AMP)
PSP Element(s) G-505100-006
G-501700-003
G-504600-004
DOI 10.1007/s00216-008-2547-1
Scopus ID 59849115160
PubMed ID 19096832
Erfassungsdatum 2009-07-09
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