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Open Access Green as soon as Postprint is submitted to ZB.
A new procalcitonin optical immunosensor for POCT applications.
Anal. Bioanal. Chem. 393, 1183-1190 (2009)
A new immunosensor for the determination of procalcitonin was developed. A sandwich assay format was implemented on a polymethylmetacrylate optical biochip, opportunely shaped in order to obtain several flow channels and potentially suitable for point of care testing applications. The sandwich format makes use of two new rat monoclonal antibodies. The capture antibody was covalently immobilised on the surface of the plastic chip, and the detection antibody was labelled with DY647 dye. Different combinations of capture and detection antibodies were investigated, and particular attention was devoted in order to avoid the nonspecific adsorption. A limit of detection of 0.088 mg L-1 was achieved within the working range of 0.28-50 mg L-1 in buffer samples. The assay was also implemented in human serum, and 0.2 and 0.7-25 mg L-1 were the attained limit of detection and working range, respectively.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Optical biochip; Immunosensor; Procalcitonin; PMMA; Fluorescence anisotropy; POCT; serum procalcitonin; infection; bacterial; sepsis
ISSN (print) / ISBN
1618-2642
e-ISSN
1618-2650
Quellenangaben
Volume: 393,
Issue: 4,
Pages: 1183-1190
Publisher
Springer
Publishing Place
Heidelberg
Reviewing status
Peer reviewed
Institute(s)
Institute of Ecological Chemistry (IOEC)
Institute of Molecular Immunology (IMI)
Research Unit Microbe-Plant Interactions (AMP)
Institute of Molecular Immunology (IMI)
Research Unit Microbe-Plant Interactions (AMP)