PuSH - Publication Server of Helmholtz Zentrum München

Ward, R.J.* ; Wilmet, S.* ; Legssyer, R.* ; Leroy, D.* ; Toussaint, L.* ; Crichton, R.R.* ; Pierreux, C.* ; Hue, L.* ; Piette, J.* ; Srai, S.K.* ; Solanky, N.* ; Klein, D. ; Summer, K.H.

Effects of marginal iron overload on iron homeostasis and immune function in alveolar macrophages isolated from pregnant and normal rats.

Biometals 22, 211-223 (2009)
DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
The effects of changes in macrophage iron status, induced by single or multiple iron injections, iron depletion or pregnancy, on both immune function and mRNA expression of genes involved in iron influx and egress have been evaluated. Macrophages isolated from iron deficient rats, or pregnant rats at day 21 of gestation, either supplemented with a single dose of iron dextran, 10 mg, at the commencement of pregnancy, or not, showed significant increases of macrophage ferroportin mRNA expression, which was paralleled by significant decreases in hepatic Hamp mRNA expression. IRP activity in macrophages was not significantly altered by iron status or the inducement of pregnancy +/- a single iron supplement. Macrophage immune function was significantly altered by iron supplementation and pregnancy. Iron supplementation, alone or combined with pregnancy, increased the activities of both NADPH oxidase and nuclear factor kappa B (NF kappa B). In contrast, the imposition of pregnancy reduced the ability of these parameters to respond to an inflammatory stimuli. Increasing iron status, if only marginally, will reduce the ability of macrophages to mount a sustained response to inflammation as well as altering iron homeostatic mechanisms.
Altmetric
Additional Metrics?
[➜Log in]
Edit extra informations Login
Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Iron homeostasis; Pregnancy; Macrophages; NF kappa B; Nitric oxide synthase; kappa-b activation; in-vivo; lipid-peroxidation; oxidative stress; nadph oxidase; expression; hepcidin; supplementation; absorption; placenta
ISSN (print) / ISBN 0966-0844
e-ISSN 1572-8773
Journal BioMetals
Quellenangaben Volume: 22, Issue: 2, Pages: 211-223 Article Number: , Supplement: ,
Publisher Springer
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Molecular Toxicology and Pharmacology (TOXI)