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Open Access Green as soon as Postprint is submitted to ZB.
Ozone-induced gene expression occurs via ethylene-dependent and -independent signalling.
Plant Mol. Biol. 51, 599-607 (2003)
Recent studies suggest that ethylene is involved in signalling ozone-induced gene expression. We show here that application of ozone increased glucuronidase (GUS) expression of chimeric reporter genes regulated by the promoters of the tobacco class I beta-1,3-glucanases (GLB and Gln2) and the grapevine resveratrol synthase (Vst1) genes in transgenic tobacco leaves. 5'-deletion analysis of the class I beta-1,3-glucanase promoter revealed that ozone-induced gene regulation is mainly mediated by the distal enhancer region containing the positively acting ethylene-responsive element (ERE). In addition, application of 1-methylcyclopropene (1-MCP), an inhibitor of ethylene action, blocked ozone-induced class I beta-1,3-glucanase promoter activity. Enhancer activity and ethylene-responsiveness depended on the integrity of the GCC boxes, cis-acting elements present in the ERE of the class I beta-1,3-glucanase and the basic-type pathogenesis-related PR-1 protein (PRB-1b) gene promoters. The minimal PRB-1b promoter containing only the ERE with intact GCC boxes, was sufficient to confer 10-fold ozone inducibility to a GUS-reporter gene, while a substitution mutation in the GCC box abolished ozone responsiveness. The ERE region of the class I beta-1,3-glucanase promoter containing two intact GCC boxes confered strong ozone inducibility to a minimal cauliflower mosaic virus (CaMV) 35S RNA promoter, whereas two single-base substitution in the GCC boxes resulted in a complete loss of ozone inducibility. Taken together, these datastrongly suggest that ethylene is signalling ozone-induced expression of class I beta-l,3-glucanase and PRB-1b genes. Promoter analysis of the stilbene synthase Vst1 gene unravelled different regions for ozone and ethylene-responsiveness. Application of 1-MCP blocked ethylene-induced Vst1 induction, but ozone induction was not affected. This shows that ozone-induced gene expression occurs via at least two different signalling mechanisms and suggests an additional ethylene independent signalling pathway for ozone-induced expression of genes involved in phytoalexin biosynthesis.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
ethylene; gene regulation; ozone; pathogenesis-related; promoter; resveratrol; stilbene; BIOCHEMICAL-PLANT RESPONSES; RESVERATROL SYNTHASE PROMOTER; PATHOGENESIS-RELATED PROTEINS; PROGRAMMED CELL-DEATH; PINUS-SYLVESTRIS L.; SALICYLIC-ACID; SCOTS PINE; DISEASE RESISTANCE; STILBENE SYNTHASE; OXIDATIVE BURST
ISSN (print) / ISBN
0167-4412
e-ISSN
0167-4412
Journal
Plant Molecular Biology
Quellenangaben
Volume: 51,
Issue: 4,
Pages: 599-607
Publisher
Springer
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institute of Biochemical Plant Pathology (BIOP)