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Gündisch, S.* ; Hauck, S.M. ; Sarioglu, H. ; Schott, C.* ; Viertler, C.* ; Kap, M.* ; Schuster, T.* ; Reischauer, B.* ; Rosenberg, R.* ; Verhoef, C.* ; Mischinger, H.-J.* ; Riegman, P.* ; Zatloukal, K.* ; Becker, K.-F.*

Variability of protein and phosphoprotein levels in clinical tissue specimens during the preanalytical phase.

J. Proteome Res. 11, 5748-5762 (2012)
DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
The quality of human tissue specimens can have a significant impact on analytical data sets for biomarker research. The aim of this study was to characterize fluctuations of protein and phosphoprotein levels in human tissue samples during the preanalytical phase. Eleven intestine and 17 liver specimens were surgically resected, aliquoted, and either snap-frozen or fixed in formalin immediately or exposed to different ischemic conditions before preservation. Protein levels in the resultant samples were investigated by reverse phase protein array, Western blot analysis, and liquid chromatography-tandem mass spectrometry. Our data revealed that the degree of sensitivity of proteins and phosphoproteins to delayed preservation varied between different patients and tissue types. For example, up-regulation of phospho-p42/44 MAPK in intestine samples was seen in some patients but not in others. General trends toward up- or down-regulation of most proteins were not evident due to pronounced interpatient variability but signal intensities of only a few proteins, such as cytokeratin 18, were altered from baseline in postresection samples. In contrast, glyceraldehyde 3-phosphate dehydrogenase was found to be stable during periods of cold ischemia. Our study represents a proper approach for studying potential protein fluctuations in tissue specimens for future biomarker development programs.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Biobank ; Biomarker ; Preanalytical Phase ; Protein ; Tissue
Language english
Publication Year 2012
HGF-reported in Year 2012
ISSN (print) / ISBN 1535-3893
e-ISSN 1535-3907
Journal Journal of Proteome Research
Quellenangaben Volume: 11, Issue: 12, Pages: 5748-5762 Article Number: , Supplement: ,
Publisher American Chemical Society (ACS)
Reviewing status Peer reviewed
Institute(s) CF Metabolomics & Proteomics (CF-MPC)
POF-Topic(s) 30203 - Molecular Targets and Therapies
Research field(s) Enabling and Novel Technologies
PSP Element(s) G-505700-001
PubMed ID 23134551
DOI 10.1021/pr300560y
Scopus ID 84870930884
Erfassungsdatum 2012-11-26
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