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Frohn, A.* ; Eberl, H.C.* ; Stöhr, J.* ; Glasmacher, E. ; Rüdel, S.* ; Heissmeyer, V. ; Mann, M.* ; Meister, G.*

Dicer-dependent and -independent Argonaute2 protein interaction networks in mammalian cells.

Mol. Cell. Proteomics 11, 1442-1456 (2012)
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Argonaute (Ago) proteins interact with small regulatory RNAs such as microRNAs (miRNAs) and facilitate gene-silencing processes. miRNAs guide Ago proteins to specific mRNAs leading to translational silencing or mRNA decay. In order to understand the mechanistic details of miRNA function, it is important to characterize Ago protein interactors. Although several proteomic studies have been performed, it is not clear how the Ago interactome changes on miRNA or mRNA binding. Here, we report the analysis of Ago protein interactions in miRNA-containing and miRNA-depleted cells. Using stable isotope labeling in cell culture in conjunction with Dicer knock out mouse embryonic fibroblasts, we identify proteins that interact with Ago2 in the presence or the absence of Dicer. In contrast to our current view, we find that Ago-mRNA interactions can also take place in the absence of miRNAs. Our proteomics approach provides a rich resource for further functional studies on the cellular roles of Ago proteins.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Embryonic Stem-cells ; Quantitative Mass-spectrometry ; Messenger-rna Decay ; Translational Repression ; Microrna Biogenesis ; Stress Granules ; Mirna Targets ; Complexes ; Proteomics ; Binding
Language english
Publication Year 2012
HGF-reported in Year 2012
ISSN (print) / ISBN 1535-9476
e-ISSN 1535-9484
Journal Molecular and Cellular Proteomics
Quellenangaben Volume: 11, Issue: 11, Pages: 1442-1456 Article Number: , Supplement: ,
Publisher American Society for Biochemistry and Molecular Biology
Reviewing status Peer reviewed
Institute(s) Institute of Molecular Immunology (IMI)
Institute of Diabetes and Obesity (IDO)
POF-Topic(s) 30504 - Mechanisms of Genetic and Environmental Influences on Health and Disease
30502 - Diabetes: Pathophysiology, Prevention and Therapy
Research field(s) Immune Response and Infection
Helmholtz Diabetes Center
PSP Element(s) G-501792-001
G-553100-001
PubMed ID 22918229
DOI 10.1074/mcp.M112.017756
WOS ID WOS:000313277100026
Scopus ID 84869237206
Erfassungsdatum 2012-12-06
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