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Open Access Green as soon as Postprint is submitted to ZB.
The zebrafish cerebellar upper rhombic lip generates tegmental hindbrain nuclei by long-distance migration in an evolutionary conserved manner.
J. Comp. Neurol. 518, 2794-2817 (2010)
The upper rhombic lip (URL) of the developing mammalian cerebellum produces different neuronal cell types in a temporal sequence. The first neuronal populations arising from this proliferation zone include the progenitors of the parabrachial, parabigeminal, and laterodorsal-pedunculopontine tegmental hindbrain nuclei. By means of expression analysis, histology, and retrograde neuronal tracing, we have identified the zebrafish homologues of these nuclei, namely, the secondary gustatory/viscerosensory nucleus, the nucleus isthmi, and the superior reticular nucleus, respectively, in the embryonic and larval brain of a stable transgenic wnt1:Gal4-VP16-14 x UAS:GFP zebrafish strain. Combining time-lapse confocal imaging with individual cell tracing, we characterize the migratory behavior of these neuronal precursor populations in detail by revealing their migration path, velocity, and directionality. In addition, we identify neuronal progenitors of the secondary gustatory/viscerosensory nucleus and nucleus isthmi/superior reticular nucleus as belonging to the polysialic acid (PSA)-expressing cell population in the cerebellar plate that migrates in a PSA-dependent manner. Finally, we reveal that circuitries involved in the processing of sensory information (visual, gustatory, general viscerosensory) are already established in the zebrafish larva at day 4 of development. Also the wnt1-expressing pretectal neuronal precursors (not originating from the URL) sending mossy fiber-like projections into the cerebellar corpus are established at that time. In sum, our results show that the origin of neurons of some tegmental hindbrain nuclei, namely, nucleus isthmi/superior reticular nucleus and secondary gustatory/viscerosensory nucleus is in the URL, and that the temporal order of cell types produced by the URL and their developmental program are conserved among vertebrate species.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Zebrafish; Neuronal migration; Cell tracing; Cerebellum; Upper rhombic lip (URL); Bioimaging; Secondary gustatory nucleus (SGN); Secondary viscerosensory nucleus (SVN); Nucleus isthmi (NI); Superior reticular nucleus (SRN); Parabrachial nuclear complex; Parabigeminal nucleus (PBG); Laterodorsal-pedunculopontine complex; Paracommissural nucleus (PC); Dorsal periventricular pretectal nucleus (PP)
ISSN (print) / ISBN
0021-9967
e-ISSN
1096-9861
Quellenangaben
Volume: 518,
Issue: 14,
Pages: 2794-2817
Publisher
Wiley
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institute of Developmental Genetics (IDG)