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Open Access Green as soon as Postprint is submitted to ZB.
A comparison of the accuracy of Illumina HumanHT-12 v3 Expression BeadChip and TaqMan qRT-PCR gene expression results in patient samples from the Tampere Vascular Study.
Atherosclerosis 226, 149-152 (2013)
Aims: This study was set up to compare the accuracy, sensitivity and specificity of gene expression results between the Illumina HumanHT-12 v3 Expression BeadChip (GWE) and the TaqMan qRT-PCR low-density array (LDA) in atherosclerotic plaques. Methods: Gene expression levels of 196 genes were determined from 22 atherosclerotic samples and 6 controls with both the GWE and the LDA. Results: The accuracies of GWE in comparison to that of qRT-PCR for absolute fold changes (FC) 1.2,1.6, 2.0 and 3.0 between cases and controls were 73.5%, 79.1%, 72.4% and 60.7%, respectively. The correlation of expression measurements between these methods was good (r = 0.87, y = 0.151 + 0.586x), and the Bland-Altman plot showed that for highly up-or down-regulated transcripts, GWE yields lower absolute FC values than LDA. Conclusion: Gene expression results obtained with the GWE are replicated with high accuracy in LDA, even for technically demanding atherosclerotic samples.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Atherosclerosis ; Genome-wide Expression Array ; Polymerase Chain Reaction ; Accuracy ; Sensitivity ; Specificity; Human Atherosclerotic Plaques ; Quality-control Maqc ; Real-time Pcr ; Microarrays
ISSN (print) / ISBN
0021-9150
e-ISSN
1879-1484
Journal
Atherosclerosis
Quellenangaben
Volume: 226,
Issue: 1,
Pages: 149-152
Publisher
Elsevier
Publishing Place
Amsterdam
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Research Unit Molecular Epidemiology (AME)