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Scherthan, H.* ; Hieber, L. ; Braselmann, H. ; Meineke, V.* ; Zitzelsberger, H.

Accumulation of DSBs in gamma-H2AX domains fuel chromosomal aberrations.

Biochem. Biophys. Res. Commun. 371, 694-697 (2008)
DOI
Open Access Green as soon as Postprint is submitted to ZB.
DNA double strand breaks (DSBs) pose a severe hazard to the genome as erroneous rejoining of DSBs can lead to mutation and cancer. Here, we have investigated the correlation between X irradiation-induced gamma-H2AX foci, theoretically induced DSBs, and the minimal number of mis-rejoined DNA breaks (MNB) in irradiated lymphocytes obtained from two healthy humans by painting of the whole chromosome complement by spectral karyotyping. There were less gamma-H2AX foci/dose than theoretically expected, while misrepair, as expressed by MNB/gamma-H2AX focus, was similar at 0.5 and 1Gy but 3.6-fold up at 3Gy. Hence, our results suggest that X-ray-induced gamma-H2AX foci in G0 lymphocyte nuclei contain more than one DSB and that the increasing number of DSBs per gamma-H2AX repair factory lead to an increased rate of misrepair.
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Publication type Article: Journal article
Document type Scientific Article
Keywords gamma-H2AX; Chromosome aberrations; DNA repair; PAINT; Ionizing radiation; Spectral karyotyping
Language english
Publication Year 2008
HGF-reported in Year 2008
ISSN (print) / ISBN 0006-291X
e-ISSN 1090-2104
Journal Biochemical and Biophysical Research Communications
Quellenangaben Volume: 371, Issue: 4, Pages: 694-697 Article Number: , Supplement: ,
Publisher Elsevier
Reviewing status Peer reviewed
Institute(s) Institute of Molecular Radiation Biology (IMS)
PSP Element(s) G-500400-002
DOI 10.1016/j.bbrc.2008.04.127
Scopus ID 44149117457
Erfassungsdatum 2008-08-22
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