PuSH - Publication Server of Helmholtz Zentrum München

Costa, M.R. ; Bucholz, O. ; Schroeder, T. ; Götz, M.

Late origin of glia-restricted progenitors in the developing mouse cerebral cortex.

Cereb. Cortex 19, (Suppl.1), i35-i143 (2009)
DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
In order to unravel the molecular determinants of cell fate, it is important to understand when fate restriction occurs during brain development. Lineage analysis suggested that bi- or multipotent progenitors persist into late developmental stages in some central nervous system regions, whereas most progenitor cells in the cerebral cortex appeared to be restrained to generate only a single cell type already at early stages. Here we discuss this previous work and present new data demonstrating that cortical progenitors generating exclusively glial cells appear late in development. In utero transduction of cortical progenitors at early and mid-neurogenesis using a combination of replication-defective retroviral vectors encoding different fluorescent proteins indicated that the early developing cortex is devoid of glia-restricted progenitors, although these are frequent during mid- and late neurogenesis. Clonal analyses in vitro using retroviral vectors and live cell tracking by video time-lapse microscopy confirmed these findings, revealing that the early developing cortex harbors 2 main progenitor types: neuron-restricted and bipotent (neuron-glial) progenitors. The latter are responsible for the generation of glial-restricted progenitors at mid- and late neurogenesis.
Altmetric
Additional Metrics?
[➜Log in]
Edit extra informations Login
Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords clonal analysis; cortical development; gliogenesis; neurogenesis; video microscopy; neural stem-cells; cortical neurogenesis; fate specification; nervous-system; precursor cell; in-vitro; neurons; lineages; generation; dispersion
ISSN (print) / ISBN 1047-3211
e-ISSN 1460-2199
Journal Cerebral Cortex
Quellenangaben Volume: 19, Issue: SUPPL. 1, Pages: i35-i143, Article Number: , Supplement: (Suppl.1)
Publisher Oxford University Press
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Stem Cell Research (ISF)