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Open Access Gold as soon as Publ. Version/Full Text is submitted to ZB.
Mesenchymal stem cells promote oligodendroglial differentiation in hippocampal slice cultures.
Cell. Physiol. Biochem. 24, 317-324 (2009)
We have previously shown that soluble factors derived from mesenchymal stem cells (MSCs) induce oligodendrogenic fate and differentiation in adult rat neural progenitors (NPCs) in vitro. Here, we investigated if this pro-oligodendrogenic effect is maintained after cells have been transplanted onto rat hippocampal slice cultures, a CNS-organotypic environment. We first tested whether NPCs, that were pre-differentiated in vitro by MSC-derived conditioned medium, would generate oligodendrocytes after transplantation. This approach resulted in the loss of grafted NPCs, suggesting that oligodendroglial pre-differentiated cells could not integrate in the tissue and therefore did not survive grafting. However, when NPCs together with MSCs were transplanted in situ into hippocampal slice cultures, the grafted NPCs survived and the majority of them differentiated into oligodendrocytes. In contrast to the prevalent oligodendroglial differentiation in case of the NPC/MSC co-transplantation, naive NPCs transplanted in the absence of MSCs differentiated predominantly into astrocytes. In summary, the pro-oligodendrogenic activity of MSCs was maintained only after co-transplantation into hippocampal slice cultures. Therefore, in the otherwise astrogenic milieu, MSCs established an oligodendrogenic niche for transplanted NPCs, and thus, co-transplantation of MSCs with NPCs might provide an attractive approach to re-myelinate the various regions of the diseased CNS.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Mesenchymal stem cells; Neural progenitors; Oligodendrocyte differentiation; Hippocampal slice culture; Multiple Sclerosis; central-nervous-system; organotypic cultures; remyelination; transplantation; proliferation; repair; model; immunosuppression; demyelination; disease
ISSN (print) / ISBN
1015-8987
e-ISSN
1421-9778
Quellenangaben
Volume: 24,
Issue: 3-4,
Pages: 317-324
Publisher
Karger
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institute of Stem Cell Research (ISF)