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Pirngruber, J.* ; Shchebet, A.* ; Morris, A.A.* ; Shema, E.* ; Minsky, N.* ; Chapman, R.D. ; Eick, D. ; Aylon, Y.* ; Oren, M.* ; Johnsen, S.A.*

CDK9 directs H2B monoubiquitination and controls replication-dependent histone mRNA 3'-end processing.

EMBO Rep. 10, 894-900 (2009)
DOI PMC
Open Access Gold as soon as Publ. Version/Full Text is submitted to ZB.
Post-translational histone modifications have essential roles in controlling nuclear processes; however, the specific mechanisms regulating these modifications and their combinatorial activities remain elusive. Cyclin-dependent kinase 9 (CDK9) regulates gene expression by phosphorylating transcriptional regulatory proteins, including the RNA polymerase II carboxy-terminal domain. Here, we show that CDK9 activity is essential for maintaining global and gene-associated levels of histone H2B monoubiquitination (H2Bub1). Furthermore, CDK9 activity and H2Bub1 help to maintain correct replication-dependent histone messenger RNA (mRNA) 3'-end processing. CDK9 knockdown consistently resulted in inefficient recognition of the correct mRNA 3'-end cleavage site and led to increased read-through of RNA polymerase II to an alternative downstream polyadenylation signal. Thus, CDK9 acts to integrate phosphorylation during transcription with chromatin modifications to control co-transcriptional histone mRNA processing.
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Publication type Article: Journal article
Document type Scientific Article
Keywords cyclin-dependent kinase; histone; mRNA processing; monoubiquitination; RNA polymerase II C-terminal domain; gene-expression; polymerase-ii; breast-cancer; transcription; chromatin; carcinomas
Language english
Publication Year 2009
HGF-reported in Year 0
ISSN (print) / ISBN 1469-221X
e-ISSN 1469-3178
Journal EMBO Reports
Quellenangaben Volume: 10, Issue: 8, Pages: 894-900 Article Number: , Supplement: ,
Publisher EMBO Press
Reviewing status Peer reviewed
Institute(s) Institute of Clinical Molecular Biology and Tumor Genetics (K.MOLBI)
POF-Topic(s) 30504 - Mechanisms of Genetic and Environmental Influences on Health and Disease
Research field(s) Immune Response and Infection
PSP Element(s) G-501400-001
PubMed ID 19575011
DOI 10.1038/embor.2009.108
Erfassungsdatum 2009-09-03
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