PuSH - Publication Server of Helmholtz Zentrum München: CDK9 directs H2B monoubiquitination and controls replication-dependent histone mRNA 3'-end processing.

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Pirngruber, J.* ; Shchebet, A.* ; Morris, A.A.* ; Shema, E.* ; Minsky, N.* ; Chapman, R.D. ; Eick, D. ; Aylon, Y.* ; Oren, M.* ; Johnsen, S.A.*

CDK9 directs H2B monoubiquitination and controls replication-dependent histone mRNA 3'-end processing.

EMBO Rep. 10, 894-900 (2009)

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Open Access Green as soon as Postprint is submitted to ZB.

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Post-translational histone modifications have essential roles in controlling nuclear processes; however, the specific mechanisms regulating these modifications and their combinatorial activities remain elusive. Cyclin-dependent kinase 9 (CDK9) regulates gene expression by phosphorylating transcriptional regulatory proteins, including the RNA polymerase II carboxy-terminal domain. Here, we show that CDK9 activity is essential for maintaining global and gene-associated levels of histone H2B monoubiquitination (H2Bub1). Furthermore, CDK9 activity and H2Bub1 help to maintain correct replication-dependent histone messenger RNA (mRNA) 3'-end processing. CDK9 knockdown consistently resulted in inefficient recognition of the correct mRNA 3'-end cleavage site and led to increased read-through of RNA polymerase II to an alternative downstream polyadenylation signal. Thus, CDK9 acts to integrate phosphorylation during transcription with chromatin modifications to control co-transcriptional histone mRNA processing.

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Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

Keywords cyclin-dependent kinase; histone; mRNA processing; monoubiquitination; RNA polymerase II C-terminal domain; gene-expression; polymerase-ii; breast-cancer; transcription; chromatin; carcinomas

ISSN (print) / ISBN 1469-221X

e-ISSN 1469-3178

Journal EMBO Reports

Quellenangaben Volume: 10, Issue: 8, Pages: 894-900

Publisher EMBO Press

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Institute of Clinical Molecular Biology and Tumor Genetics (K.MOLBI)