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Antranikian, G.* ; Ruepp, A. ; Gordon, P.M.* ; Ballschmiter, M.* ; Zibat, A.* ; Stark, M.* ; Sensen, C.W.* ; Frishman, D.* ; Liebl, W.* ; Klenk, H.P.*

Rapid access to genes of biotechnologically useful enzymes by partial genome sequencing: The thermoalkaliphile Anaerobranca gottschalkii.

J. Mol. Microbiol. Biotechnol. 16, 81-90 (2009)
DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
Anaerobranca gottschalkii strain LBS3 T is an extremophile living at high temperature (up to 65 degrees C) and in alkaline environments (up to pH 10.5). An assembly of 696 DNA contigs representing about 96% of the 2.26-Mbp genome of A. gottschalkii has been generated with a low-sequence-coverage shotgun-sequencing strategy. The chosen sequencing strategy provided rapid and economical access to genes encoding key enzymes of the mono- and polysaccharide metabolism, without dilution of spare resources for extensive sequencing of genes lacking potential economical value. Five of these amylolytic enzymes of considerable commercial interest for biotechnological applications have been expressed and characterized in more detail after identification of their genes in the partial genome sequence: type I pullulanase, cyclodextrin glycosyltransferase (CGTase), two alpha-amylases (AmyA and AmyB), and an alpha-1,4-glucan-branching enzyme.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Rapid access to genes; Biotechnologically useful enzymes; Partial genome sequencing; Anaerobranca gottschalkii; Amylolytic enzymes
Language english
Publication Year 2009
HGF-reported in Year 2009
ISSN (print) / ISBN 1464-1801
e-ISSN 1660-2412
Journal Journal of Molecular Microbiology and Biotechnology
Quellenangaben Volume: 16, Issue: 1-2, Pages: 81-90 Article Number: , Supplement: ,
Publisher Karger
Reviewing status Peer reviewed
Institute(s) Institute of Bioinformatics and Systems Biology (IBIS)
POF-Topic(s) 30505 - New Technologies for Biomedical Discoveries
Research field(s) Enabling and Novel Technologies
PSP Element(s) G-503700-001
PubMed ID 18957864
DOI 10.1159/000142896
Scopus ID 55249113737
Erfassungsdatum 2009-09-21
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