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Open Access Green as soon as Postprint is submitted to ZB.
Rapid access to genes of biotechnologically useful enzymes by partial genome sequencing: The thermoalkaliphile Anaerobranca gottschalkii.
J. Mol. Microbiol. Biotechnol. 16, 81-90 (2009)
Anaerobranca gottschalkii strain LBS3 T is an extremophile living at high temperature (up to 65 degrees C) and in alkaline environments (up to pH 10.5). An assembly of 696 DNA contigs representing about 96% of the 2.26-Mbp genome of A. gottschalkii has been generated with a low-sequence-coverage shotgun-sequencing strategy. The chosen sequencing strategy provided rapid and economical access to genes encoding key enzymes of the mono- and polysaccharide metabolism, without dilution of spare resources for extensive sequencing of genes lacking potential economical value. Five of these amylolytic enzymes of considerable commercial interest for biotechnological applications have been expressed and characterized in more detail after identification of their genes in the partial genome sequence: type I pullulanase, cyclodextrin glycosyltransferase (CGTase), two alpha-amylases (AmyA and AmyB), and an alpha-1,4-glucan-branching enzyme.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Rapid access to genes; Biotechnologically useful enzymes; Partial genome sequencing; Anaerobranca gottschalkii; Amylolytic enzymes
ISSN (print) / ISBN
1464-1801
e-ISSN
1660-2412
Quellenangaben
Volume: 16,
Issue: 1-2,
Pages: 81-90
Publisher
Karger
Non-patent literature
Publications
Reviewing status
Peer reviewed