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Panthel, K.* ; Meinel, K.M.* ; Domenech, V.E.S.* ; Geginat, G.* ; Linkemann, K.* ; Busch, D.H. ; Rüssmann, H.*

Prophylactic anti-tumor immuntiy against a murine fibrosarcoma triggered by the Salmonella type III secretion system.

Microbes Infect. 8, 2539-2546 (2006)
DOI
Open Access Green as soon as Postprint is submitted to ZB.
The potential of an attenuated Salmonella enterica serovar Typhimurium strain as a prophylactic anti-tumor vaccine against the murine fibrosarcoma WEHI 164 was evaluated. Tumor cells were transfected with the DNA sequence encoding the MHC class I-restricted peptide p60(217-225) from Listeria monocytogenes. BALB/c mice received a single orogastric immunization with Salmonella that translocates a chimeric p60 protein via its type III secretion system. Mice were subsequently challenged subcutaneously with p60(217-225)-expressing WEHI cells. In vivo protection studies revealed that 80% of these mice remained free of the fibrosarcoma after challenge, whereas all animals of the non-vaccinated control group did develop tumor growth. In further experiments, the distribution of tetramer-positive p60(217-225)-specific effector and memory CD8 T cells after Salmonella-based immunization and tumor application was analyzed. Costaining with CD62L and CD127 revealed a predominance of p60-specific central memory and effector memory CD8 T cells in spleens, whereas in blood samples the majority of p60-specific lymphocytes belonged to effector and effector memory CD8 T cell subsets. This is the first report demonstrating that a bacterial type III secretion system can be used for heterologous antigen delivery to induce cytotoxic effector and memory CD8 T cell responses resulting in an efficient prevention of tumor growth.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Salmonella enterica serovar Typhimurium; Type III secretion system; tumor vaccine; CD8 T cell; memory T cell
ISSN (print) / ISBN 1286-4579
e-ISSN 1769-714X
Journal Microbes and Infection
Quellenangaben Volume: 8, Issue: 9-10, Pages: 2539-2546 Article Number: , Supplement: ,
Publisher Elsevier
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) CCG Antigen-specific Immunotherapy (VIRO-KVA)