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Open Access Green as soon as Postprint is submitted to ZB.
Photoactivation of the CreER T² recombinase for conditional site-specific recombination with high spatiotemporal resolution.
Zebrafish 7, 199-204 (2010)
We implemented a noninvasive optical method for the fast control of Cre recombinase in single cells of a live zebrafish embryo. Optical uncaging of the caged precursor of a nonendogeneous steroid by one- or two-photon illumination was used to restore Cre activity of the CreER(T2) fusion protein in specific target cells. This method labels single cells irreversibly by inducing recombination in an appropriate reporter transgenic animal and thereby can achieve high spatiotemporal resolution in the control of gene expression. This technique could be used more generally to investigate important physiological processes (e.g., in embryogenesis, organ regeneration, or carcinogenesis) with high spatiotemporal resolution (single cell and 10-min scales).
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Transgenic zebrafish; Mice; Expression; System; Tool
ISSN (print) / ISBN
1545-8547
e-ISSN
1557-8542
Journal
Zebrafish
Quellenangaben
Volume: 7,
Issue: 2,
Pages: 199-204
Publisher
Mary Ann Liebert
Reviewing status
Peer reviewed
Institute(s)
Institute of Developmental Genetics (IDG)