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Hültner, L. ; Szöts, H. ; Welle, M. ; van Snick, J. ; Moeller, J. ; Dörmer, P.

Mouse Bone Marrow-Derived IL-3-dependent Mast Cells and Autonomous Sublines Produce IL-6.

Immunology 67, 408-413 (1989)

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This study deals with the question of whether mouse bone marrow-derived mast cells are able to produce interleukin-6 (IL-6) in vitro. For this purpose, a panel of primary mast cell clones from limiting-dilution microcultures, of permanent IL-3-dependent mast cell lines and autonomous malignant sublines, was screened. All of these lines were found to produce growth factor activity for IL-6-dependent mouse hybridoma cells (7TD1), which could be completely neutralized by the monoclonal anti-IL-6-antibody 6B4. Transcriptional activity of the IL-6 gene was demonstrated in both IL-3-dependent mast cells and autonomous sublines using a mouse IL-6-specific cDNA probe.

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