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Open Access Green as soon as Postprint is submitted to ZB.
Construction and characterization of a packaging cell line for MMTV-based conditional retroviral vectors.
Biochem. Biophys. Res. Commun. 159, 1191-1198 (1989)
A chimeric provirus in which the 5'LTR of a complete biologically active Mouse Mammary Tumour Virus (MMTV) proviral DNA has been replaced with the Rous Sarcoma Virus LTR has been constructed. Upon transfection into permissive cells, this provirus directs the synthesis of the MMTV gag and env structural proteins, but is impaired in packaging of the RNAs that encode these proteins. Supertransfection of these cells with MMTV based vector constructs results in the production of infectious recombinant virus at a higher efficiency than with previously described helper cell lines. Such a retroviral vector system based on MMTV will allow the study of the effects of conditional expression of inserted genes upon infected cells.
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Publication type
Article: Journal article
Document type
Scientific Article
ISSN (print) / ISBN
0006-291X
e-ISSN
1090-2104
Quellenangaben
Volume: 159,
Issue: 3,
Pages: 1191-1198
Publisher
Elsevier
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Departments & Institutes