OpenSSL SSL_connect: Connection reset by peer in connection to v2.sherpa.ac.uk:443 PuSH - Publication Server of Helmholtz Zentrum München: A novel cytosolic class I antigen-processing pathway for endoplasmic-reticulum-targeted proteins.

PuSH - Publication Server of Helmholtz Zentrum München

Schlosser, E.* ; Otero, C.* ; Wuensch, C.* ; Kessler, B.* ; Edelmann, M.* ; Brunisholz, R.* ; Drexler, I. ; Legler, D.F.* ; Groettrup, M.

A novel cytosolic class I antigen-processing pathway for endoplasmic-reticulum-targeted proteins.

EMBO Rep. 8, 945-951 (2007)
DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
Proteins bearing an endoplasmic reticulum (ER) leader are inserted into the ER followed by cleavage of the signal peptide. Major histocompatibility complex class I-restricted T-cell epitopes can be generated from these proteins by the proteasome after retrotranslocation into the cytosol. Here, we show that an HLA-A*0201-restricted epitope from prostate stem cell antigen contains the cleavage site of the ER signal peptidase. The resulting cleavage products fail to bind to HLA-A*0201 and are not recognized by T lymphocytes. As processing of prostate stem cell antigen by signal peptidase occurs immediately after co-translational insertion, the epitope must be processed from polypeptides that have never reached the ER. The processing of this epitope depends on the proteasome and the transporter associated with antigen processing and shows a novel pathway of class I processing that relies on the failure of ER-targeted proteins to reach their target compartment.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords endoplasmic reticulum; antigen processing; Major histocompatibility complex class I; signal peptidase; cytoxic T lymphocytes
ISSN (print) / ISBN 1469-221X
e-ISSN 1469-3178
Journal EMBO Reports
Quellenangaben Volume: 8, Issue: 10, Pages: 945-951 Article Number: , Supplement: ,
Publisher EMBO Press
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Virology (VIRO)