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Open Access Gold as soon as Publ. Version/Full Text is submitted to ZB.
Enhanced CD95-mediated apoptosis contributes to radiation hypersensitivity of NBS lymphoblasts.
Apoptosis 12, 753-767 (2007)
The molecular causes for enhanced radiosensitivity of Nijmegen Breakage Syndrome cells are unclear, especially as repair of DNA damage is hardly impeded in these cells. We clearly demonstrate that radiation hypersensitivity is accompanied by enhanced gamma-radiation-induced apoptosis in NBS1 deficient lymphoblastoid cell lines. Differences in the apoptotic behavior of NBS1 (-/-) and NBS1 (+/-) cells are not due to an altered p53 stabilization or phosphorylation in NBS1 (-/-) cells. gamma-radiation-induced caspase-8 activity is increased and visualization of CD95 clustering by laser scanning microscopy shows a significant higher activation of the death receptor in NBS1 (-/-) cells. Further investigation of the molecular mechanisms reveals a role for reactive oxygen species-triggered activation of CD95. These results demonstrate that NBS1 suppresses the CD95 death receptor-dependent apoptotic pathway after gamma-irradiation and evidence is given that this is achieved by regulation of the PI3-K/AKT survival pathway.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Apoptosis; CD95; gamma-radiation; NBS1; Radiation hypersensitivity; PI3-kinase
ISSN (print) / ISBN
1360-8185
e-ISSN
1573-675X
Journal
Apoptosis
Quellenangaben
Volume: 12,
Issue: 4,
Pages: 753-767
Publisher
Springer
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institute of Radiation Biology (ISB)