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Egloff, S.* ; O'Reilly, D.* ; Chapman, R.D. ; Taylor, A.* ; Tanzhaus, K.* ; Pitts, L.* ; Eick, D. ; Murphy, S.*

Serine-7 of the RNA polymerase II CTD is specifically required for snRNA gene expression.

Science 318, 1777-1779 (2007)
Postprint DOI PMC
Open Access Green
RNA polymerase II (Pol II) transcribes genes that encode proteins and noncoding small nuclear RNAs (snRNAs). The carboxyl-terminal repeat domain (CTD) of the largest subunit of mammalian RNA Pol II, comprising tandem repeats of the heptapeptide consensus Tyr1-Ser2-Pro3-Thr4-Ser5-Pro6-Ser7, is required for expression of both gene types. We show that mutation of serine-7 to alanine causes a specific defect in snRNA gene expression. We also present evidence that phosphorylation of serine-7 facilitates interaction with the snRNA gene-specific Integrator complex. These findings assign a biological function to this amino acid and highlight a gene type-specific requirement for a residue within the CTD heptapeptide, supporting the existence of a CTD code.
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Publication type Article: Journal article
Document type Scientific Article
ISSN (print) / ISBN 0036-8075
e-ISSN 1095-9203
Journal Science
Quellenangaben Volume: 318, Issue: 5857, Pages: 1777-1779 Article Number: , Supplement: ,
Publisher American Association for the Advancement of Science (AAAS)
Reviewing status Peer reviewed
Institute(s) Institute of Functional Epigenetics (IFE)
Institute of Clinical Molecular Biology and Tumor Genetics (K.MOLBI)