PuSH - Publication Server of Helmholtz Zentrum München: Flow cytometric analysis of G<sub>1</sub>- and G<sub>2</sub>/M-phase subpopulations in mammalian cell nuclei using side scatter and DNA content measurements.

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Nüsse, M. ; Beisker, W. ; Hoffmann, C. ; Tarnok, A.

Flow cytometric analysis of G₁- and G₂/M-phase subpopulations in mammalian cell nuclei using side scatter and DNA content measurements.

Cytometry A 11, 813-821 (1990)

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Several subcompartments of the cell cycle in addition to the G₁-, S-, and G₂-phases usually observed were identified by simultaneous flow cytometric measurements of ethidium bromide fluorescence and side scatter intensity of cell nuclei. Metaphase cells and very early G₁-phase cells (G₁A) with low side scatter intensities were discriminated from interphase cells with high side scatter intensities. The reason for the various side scatter intensities was found to be the different structure of metaphase cells and early G₁-phase cells due to chromatin condensation as shown by sorting of the respective cell nuclei. The G₁A-phase could further be subdivided into two compartments with very low side scatter (G₁A₁) and intermediate side scatter (G₁A₂) intensities. Using partially synchronized cells the duration of these subcompartments of the G₁-phase could be estimated. The durations of G₁A₁- and G₁A₂-phases were found to be about 10 min and 20 min, respectively, compared to the total duration of the G₁-phase of about 3 h. Additional flow cytometric measurements of side scatter intensities of cell nuclei provide therefore further.

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PuSH - Publication Server of Helmholtz Zentrum München

Flow cytometric analysis of G1- and G2/M-phase subpopulations in mammalian cell nuclei using side scatter and DNA content measurements.

Flow cytometric analysis of G₁- and G₂/M-phase subpopulations in mammalian cell nuclei using side scatter and DNA content measurements.