PuSH - Publication Server of Helmholtz Zentrum München: Two-dimensional Electrophoresis of Dog Bronchoalveolar Lavage Fluid Proteins.

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Lenz, A.-G. ; Meyer, B. ; Weber, H. ; Maier, K.L.

Two-dimensional Electrophoresis of Dog Bronchoalveolar Lavage Fluid Proteins.

Electrophoresis 11, 510-513 (1990)

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Open Access Green as soon as Postprint is submitted to ZB.

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Proteins of dog bronchoalveolar lavage fluid, obtained by washing the epithelial lining layer of lungs with phosphate-buffered saline, were separated by two-dimensional electrophoresis. Due to the low protein and high salt content of the bronchoalveolar lavage fluid, samples had to be concentrated and desalted. Following electrophoresis the protein spots were visualized by silver staining. Comparing the two-dimensional protein patterns of bronchoalveolar lavage fluid with that from serum, several lungspecific proteins were detected. The most prominent protein, most probably a surfactant-associated protein, showed isoforms with isoelectric points in the range of pH 4.2–4.8, and a molecular mass of 32 kDa on sodium dodecyl sulfate-polyacrylamide gel electrophoresis after reduction with dithiothreitol.

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