PuSH - Publication Server of Helmholtz Zentrum München

Export: TXT Text RIS Endnote (RIS) BIB BibTeX
Steuber-Buchberger, P.M. ; Wurst, W. ; Kühn, R.

Simultaneous Cre-mediated conditional knockdown of two genes in mice.

Genesis 46, 144-151 (2008)
DOI
Open Access Green as soon as Postprint is submitted to ZB.
One of the most promising techniques to manipulate gene expression in vivo is the use of RNA interference (RNAi). Various approaches were developed to use RNAi in the mouse, including vector-based expression of short hairpin RNAs and the Cre/loxP recombination system. We combined these two approaches to create a vector system that allows the time- and tissue-specific control of two genes at the same time. For this purpose two independent conditional shRNA expression cassettes are combined into a single construct. By the use of different, incompatible pairs of lox sites that flank transcriptional stop cassettes, Cre recombinase can independently activate both short hairpins. Here, we show that Cre simultaneously activates both shRNAs in vitro and in vivo. We applied this technique to silence the widely coexpressed gene pairs Gsk-3 alpha/Gsk-3 beta and Erk1/Erk2 in murine embryonic stem cells and in the mouse brain.
Impact Factor
Scopus SNIP
Web of Science
Times Cited
Scopus
Cited By
Altmetric
2.615
0.000
23
26
Tags
Annotations
Special Publikation
Hide on homepage

Edit extra information
Edit own tags
Private
Edit own annotation
Private
Hide on publication lists
on hompage
Mark as special
publikation
Publication type Article: Journal article
Document type Scientific Article
Keywords shRNA; recombinase mediated cassette exchange; adult mouse brain; GSK-3
Language
Publication Year 2008
HGF-reported in Year 2008
ISSN (print) / ISBN 1526-954X
e-ISSN 1526-968X
Journal Genesis : The Journal of Genetics and Development
Quellenangaben Volume: 46, Issue: 3, Pages: 144-151 Article Number: , Supplement: ,
Publisher Wiley
Reviewing status Peer reviewed
Institute(s) Institute of Developmental Genetics (IDG)
POF-Topic(s) 30204 - Cell Programming and Repair
Research field(s) Genetics and Epidemiology
PSP Element(s) G-500500-001
DOI 10.1002/dvg.20376
Scopus ID 42349112418
Erfassungsdatum 2008-12-31
[➜Report correction]