PuSH - Publication Server of Helmholtz Zentrum München: Centromere Detection in Vinblastine- and Radiation-induced Micronuclei of Cytokinesis-blocked Mouse Cells Using in Situ Hybridization with a Mouse gamma (major) Satellite DNA Probe.

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Salassidis, K. ; Huber, R. ; Zitzelsberger, H. ; Bauchinger, M.

Centromere Detection in Vinblastine- and Radiation-induced Micronuclei of Cytokinesis-blocked Mouse Cells Using in Situ Hybridization with a Mouse gamma (major) Satellite DNA Probe.

Environ. Mol. Mutagen. 19, 1-6 (1992)

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Non-isotopic in situ hybridization using a mouse gamma (major) satellite probe DNA was applied to detect centromeres in micronuclei, which were induced in vitro in mouse liver cells by ionizing radiation and by vinblastine sulfate. In a cytokinesis-blocked micronucleus assay a dose-dependent induction of micronuclei was found for both agents. After vinblastine exposure the observed micronuclei showed centromere-positive hybridization signals in an order of magnitude of 70-90%, but after radiation exposure the magnitude was only 10-20%. Since the in situ hybridization technique detects centromeric DNA directly, it can be used in a cytokinesis-blocked micronucleus assay for a rapid and reliable discrimination between aneuploidy-inducing and clastogenic agents.

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Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

ISSN (print) / ISBN 0192-2521

e-ISSN 1098-2280

Journal Environmental and Molecular Mutagenesis

Quellenangaben Volume: 19, Issue: 1, Pages: 1-6

Publisher Wiley

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Institute of Radiation Biology (ISB)