OpenSSL SSL_connect: Connection reset by peer in connection to v2.sherpa.ac.uk:443 PuSH - Publication Server of Helmholtz Zentrum München: Tandem affinity purification of protein complexes from mammalian cells by the Strep/FLAG (SF)-TAP tag.

PuSH - Publication Server of Helmholtz Zentrum München

Gloeckner, C.J. ; Boldt, K. ; Schumacher, A.* ; Ueffing, M.

Tandem affinity purification of protein complexes from mammalian cells by the Strep/FLAG (SF)-TAP tag.

Methods Mol. Biol. 564, 359-372 (2009)
DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
Isolation and dissection of native multiprotein complexes is a central theme in functional genomics. The development of the tandem affinity purification (TAP) tag has enabled efficient and large-scale purification of native protein complexes. The SF-TAP tag, a modified version of the TAP tag, allows a fast and straightforward purification of protein complexes from mammalian cells. It consists of a tandem Strep-tag II and a FLAG epitope (SF-TAP). The SF-TAP tag allows a native elution of protein complexes without proteolytic cleavage needed in the original TAP procedure. Besides the SF-TAP protocol, the principal idea of a pathway mapping by subsequent tagging of copurified proteins is demonstrated for the interactome of the MAPKKK Raf.
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Publication type Article: Journal article
Document type Scientific Article
Editors Reinders, R.* ; Sickmann, A.*
Corresponding Author
Keywords Protein complexes; Tandem affinity tag; TAP; SF-TAP
ISSN (print) / ISBN 1064-3745
e-ISSN 1940-6029
Conference Title Proteomics : Methods and Protocols
Journal Methods in Molecular Biology
Quellenangaben Volume: 564, Issue: , Pages: 359-372 Article Number: , Supplement: ,
Series Methods in Molecular Biology
Publisher Springer
Publishing Place Berlin [u.a.]
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) CF Metabolomics & Proteomics (CF-MPC)