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In vivo imaging of CT26 mouse tumours by using cmHsp70.1 monoclonal antibody.
J. Cell. Mol. Med. 15, 874-887 (2011)
The major stress-inducible heat shock protein 70 (Hsp70) is frequently present on the cell surface of human tumors, but not on normal cells. Herein, the binding characteristics of the cmHsp70.1 mouse monoclonal antibody (mAb) were evaluated in vitro and in a syngeneic tumor mouse model. More than 50% of the CT26 mouse colon carcinoma cells express Hsp70 on their cell surface at 4 degrees C. After a temperature shift to 37 degrees C, the cmHsp70.1-FITC mAb translocates into early endosomes and lysosomes Intraoperative and near-infrared fluorescence (NIRF) imaging revealed an enrichment of Cy5.5-conjugated mAb cmHsp70.1, but not an identically labelled IgG1 isotype-matched control, in i.p. and s.c. located CT26 tumors, as soon as 30min after i.v. injection into the tail vein. Due to the rapid turnover rate of membrane-bound Hsp70, the fluorescence-labelled cmHsp70.1 mAb became endocytosed and accumulated in the tumor, reaching a maximum after 24h and remained detectable at least up to 96h after a single i.v. injection. The tumor-selective internalization of mAb cmHsp70.1 at the physiological temperature of 37 degrees C might enable a targeted uptake of toxins or radionuclides into Hsp70 membrane-positive tumors. The anti-tumoral activity of the cmHsp70.1 mAb is further supported by its capacity to mediate antibody dependent cytotoxicity (ADCC).
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
membrane-bound Hsp70; Hsp70 antibody; tumor mouse model; flat panel volume CT; near-infrared fluorescence imaging
Language
english
Publication Year
2011
Prepublished in Year
2010
HGF-reported in Year
2010
ISSN (print) / ISBN
1582-1838
e-ISSN
1582-4934
Quellenangaben
Volume: 15,
Issue: 4,
Pages: 874-887
Publisher
Blackwell
Reviewing status
Peer reviewed
Institute(s)
CCG Innate Immunity in Tumor Biology (PATH-KTB)
Institute of Biological and Medical Imaging (IBMI)
Institute of Pathology (PATH)
Research Unit Analytical Pathology (AAP)
Institute of Biological and Medical Imaging (IBMI)
Institute of Pathology (PATH)
Research Unit Analytical Pathology (AAP)
POF-Topic(s)
30504 - Mechanisms of Genetic and Environmental Influences on Health and Disease
30205 - Bioengineering and Digital Health
30205 - Bioengineering and Digital Health
Research field(s)
Enabling and Novel Technologies
Enabling and Novel Technologies
PSP Element(s)
G-521400-001
G-505500-001
G-500300-001
G-500390-001
G-505500-001
G-500300-001
G-500390-001
PubMed ID
20406322
Scopus ID
79955661839
Erfassungsdatum
2010-10-18