PuSH - Publication Server of Helmholtz Zentrum München: Accumulation of p53 Protein as an Indicator for p53 Gene Mutation in Breast Cancer: Occurence of False-Positives and False-Negatives.

Navigation

Home

Deutsch

Research

Advanced Search

Browse by ...

... Journal

... Publication Type

... Research Data

... Publication Year

Publication overview

Support & Contact

Contact persons

Help

Data protection

Lohmann, D. ; Ruhri, C. ; Schmitt, M. ; Graeff, H. ; Höfler, H.

Accumulation of p53 Protein as an Indicator for p53 Gene Mutation in Breast Cancer: Occurence of False-Positives and False-Negatives.

Diagn. Mol. Pathol. 2, 36-41 (1993)

DOI

PMC

Open Access Green as soon as Postprint is submitted to ZB.

Abstract
Metrics
Extra information

Accumulation of p53 protein resulting in levels detectable by immunohistochemistry (IHC) has been proposed as an indicator of mutation of the p53 gene. We have investigated a panel of 23 fresh-frozen breast cancers by IHC (PAb 1801), Southern and Northern blot analysis, and direct sequencing of the mutation hot spot regions (exons 5-8) of the p53 gene. Three tumors (13%) showed an intense nuclear staining in the majority of malignant cells, but only one of these showed a mutation of the p53 gene (codon 237, Arg to His). Furthermore, a mutation (5-bp deletion) was identified in a tumor that showed no p53 immunoreactivity. Our results indicate that accumulation of p53 protein, as detectable by IHC, is not a reliable indicator for p53 gene mutation in human breast cancer.

Altmetric

Additional Metrics?

[➜Log in]

Edit extra informations Login

Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

ISSN (print) / ISBN 1052-9551

e-ISSN 1533-4066

Journal Diagnostic Molecular Pathology

Quellenangaben Volume: 2, Issue: 1, Pages: 36-41

Publisher Lippincott Williams & Wilkins

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Institute of Pathology (PATH)