PuSH - Publication Server of Helmholtz Zentrum München: A new, versatile field immunosensor for environmental pollutants. Development and proof of principle with TNT, diuron and atrazine.

Navigation

Home

Deutsch

Research

Advanced Search

Browse by ...

... Journal

... Publication Type

... Research Data

... Publication Year

Publication overview

Support & Contact

Contact persons

Help

Data protection

Ciumasu, I.M.* ; Krämer, P.M. ; Weber, C.M. ; Kolb, G.* ; Tiemann, D.* ; Windisch, S.* ; Frese, I.* ; Kettrup, A.

A new, versatile field immunosensor for environmental pollutants. Development and proof of principle with TNT, diuron and atrazine.

Biosens. Bioelectron. 21, 354-364 (2005)

DOI

Open Access Green as soon as Postprint is submitted to ZB.

Abstract
Metrics
Extra information

This paper presents a new, versatile, portable miniaturized flow-injection immunosensor which is designed for field analysis. The temperature-controlled field prototype can run for 6 h without external power supply. The bio-recognition element is an analyte-specific antibody immobilized on a gold surface of pyramidal structures inside an exchangeable single-use chip, which hosts also the enzyme-tracer and the sample reservoirs. The competition between the enzyme-tracer and the analyte for the antigen-binding sites of the antibodies yields in the final step a chemiluminescence signal that is inversely proportional to the concentration of analyte in the given range of detection. A proof of principle is shown for nitroaromatics and pesticides. The detection limits (DL; IC₂₀) reached with the field prototype in the laboratory was below 0.1 μg l⁻¹ for 2,4,6-trinitrotoluene (TNT), and about 0.2 μg l⁻¹ for diuron and atrazine, respectively. Important aspects in this development were the design of the competition between analyte and enzyme-tracer, the unspecific signal due to unspecific binding and/or luminescence background signal, and the flow pattern inside the chip.

Altmetric

Additional Metrics?

[➜Log in]

Edit extra informations Login

Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

Keywords flow-injection immunosensor; chemiluminescence; nitroaromatic compounds; pesticides; TNT; atrazine; diuron; isoproturon

ISSN (print) / ISBN 0956-5663

e-ISSN 1873-4235

Journal Biosensors and Bioelectronics

Quellenangaben Volume: 21, Issue: 2, Pages: 354-364

Publisher Elsevier

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Institute of Ecological Chemistry (IOEC)