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Open Access Green as soon as Postprint is submitted to ZB.
Iodine speciation in biological samples by capillary electrophoresis : inductively coupled plasma mass spectrometry.
Electrophoresis 20, 2547-2553 (1999)
A hyphenation of capillary electrophoresis (CE) to inductively coupled plasma mass spectrometry (ICP-MS) was employed for the speciation of iodine. The separation method used a buffer sandwich of phosphate (pH 2.3), NaOH, sodium dodecyl sulfate (SDS) and borate buffer (pH 8.3) for stacking, aiming at sufficient separation of iodide, iodate, thyroxine (T4) and triiodothyronine (T3). These four iodine species were separated within 15 min and subsequently detected during a pressure-driven detection step (baseline-separated) at 19.5, 29.1, 36.6 and 42.2 s. The detection limits were determined at 0.08 μg I/L (iodide), 0.3 μg I/L (iodate), 3.5 μg I/L (thyroxine) and 2.5 μg I/L (triiodothyronine). This method was applied on iodine speciation in human serum (“healthy” and after thyroid gland operation) and urine. The serum from the healthy person contained iodide (13 μg I/L), T4 (61 μg I/L) and T3 (7.5 μg I/L), whereas the serum from the thyroid-operated person lacked T3. As no “free” I-hormones are known in serum, the role of the thyroid hormone binding globulin (TBG) was investigated. We found that spiked T4 or T3 immediately bound to TBG. Investigations on human urine showed only a peak for iodide.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Iodine speciation; Human serum; Capillary electrophoresis; Inductively coupled
plasma-mass spectrometry hyphenation
ISSN (print) / ISBN
0173-0835
e-ISSN
1522-2683
Journal
Electrophoresis
Quellenangaben
Volume: 20,
Pages: 2547-2553
Publisher
Wiley
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institute of Ecological Chemistry (IOEC)