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Nolte, A.* ; Buhmann, R.* ; Emmerich, B.* ; Schendel, D.J. ; Hallek, M.*

Reconstruction of the cellular immune response after autologous peripheral blood stem cell transplantation in patients with non-Hodgkin's lymphoma.

Br. J. Haematol. 108, 415-423 (2000)

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Open Access Green as soon as Postprint is submitted to ZB.

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Peripheral blood stem cell (PBSC) transplants may be depleted of lymphoid progenitors, thereby disabling the cellular immune response against viral pathogens after autologous PBSC transplantation (PBSCT). To monitor the cellular immune reconstitution after autologous PBSCT, we investigated the cytolytic activity (CLA) of peripheral blood T lymphocytes against Epstein–Barr virus (EBV) in 13 patients with non-Hodgkin‘s lymphoma or multiple myeloma. The individual EBV-directed CLA (EBV-CLA) was determined by calculating the number of cytolytic effector cells in 10⁶ T cells needed to lyse 25% of autologous EBV-transformed B-lymphoblastoid cells, expressed as lytic units (LU₂₅). During the first 6 months after PBSCT, the EBV-CLA was only 14.6% of the response of healthy controls (median 4.8 vs. 32.9 LU₂₅). Thereafter, the EBV-CLA increased to 28.15 LU₂₅ (median) or 86% of healthy controls. Monthly follow-up analyses in five selected patients showed that the EBV-CLA was barely detectable at 4 weeks and recovered at 8–12 weeks after PBSCT in four out of five patients. Effector cells consisted mostly of CD8-positive T lymphocytes, with small CD4- and CD3/CD56-positive lymphocyte fractions. These results suggest that the reconstitution of the cellular immune response against EBV takes 8–12 weeks after autologous PBSCT.

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Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

Keywords non-Hodgkin's lymphoma peripheral blood stem cell transplantation immune reconstruction cytotoxic T lymphocytes Epstein-Barr virus

ISSN (print) / ISBN 0007-1048

e-ISSN 1365-2141

Journal British Journal of Haematology

Quellenangaben Volume: 108, Issue: 2, Pages: 415-423

Publisher Wiley

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Institute of Molecular Immunology (IMI)