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Lengler, J. ; Graw, J.

Regulation of the Human SIX3 Gene Promoter.

Biochem. Biophys. Res. Commun. 287, 372-376 (2001)
DOI
Open Access Green as soon as Postprint is submitted to ZB.
A 2-kb promoter fragment of SIX3, a human transcription factor essential for vertebrate eye development, has been characterized in a gene reporter assay system. The peak of activity implies the 2-kb sequence of SIX3, whereas 5′-deletion constructs of the promoter decreases successively to 60% of the activity starting from the entire promoter. In contrast, cutting off 300 bp of the 3′ promoter extinguishes its activity completely. Coexpression experiments of different other transcription factors illuminate the regulation of SIX3 during eye development: Pax6 activates the −703/−349 SIX3 promoter threefold, and PROX1 even eightfold. In contrast, Msx2 represses the entire SIX3 promoter. Furthermore, Six3 is regulated by its own negative feedback loop. In conclusion, SIX3 expression underlies a complex regulation, which is an important part to understand the network of transcription factors during eye development.A 2-kb promoter fragment of SIX3, a human transcription factor essential for vertebrate eye development, has been characterized in a gene reporter assay system. The peak of activity implies the 2-kb sequence of SIX3, whereas 5′-deletion constructs of the promoter decreases successively to 60% of the activity starting from the entire promoter. In contrast, cutting off 300 bp of the 3′ promoter extinguishes its activity completely. Coexpression experiments of different other transcription factors illuminate the regulation of SIX3 during eye development: Pax6 activates the −703/−349 SIX3 promoter threefold, and PROX1 even eightfold. In contrast, Msx2 represses the entire SIX3 promoter. Furthermore, Six3 is regulated by its own negative feedback loop. In conclusion, SIX3 expression underlies a complex regulation, which is an important part to understand the network of transcription factors during eye development.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords SIX3 promoter gene reporter assay transcription factor eye development mouse man
ISSN (print) / ISBN 0006-291X
e-ISSN 1090-2104
Journal Biochemical and Biophysical Research Communications
Quellenangaben Volume: 287, Issue: 2, Pages: 372-376 Article Number: , Supplement: ,
Publisher Elsevier
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Developmental Genetics (IDG)