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Hariton-Gazal, E.* ; Feder, R.* ; Graessmann, A.* ; Brack-Werner, R. ; Jans, D.* ; Gilon, C.* ; Loyter, A.*

Targeting of Nonkaryophilic Cell-Permeable Peptides into the Nuclei of Intact Cells by Covalently Attached Nuclear Localization Signals.

Biochemistry 41, 9208-9214 (2002)

DOI

Open Access Green as soon as Postprint is submitted to ZB.

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Dermaseptins are a family of antimicrobial peptides that lyse target bacterial cells by destabilization of their membranes. Here we present a novel application of a peptide derived from the dermaseptin S4, S4₁₃. At nontoxic concentrations, fluorescently labeled S4₁₃ was able to penetrate intact cultured HeLa cells but essentially failed to enter their nuclei despite its low molecular weight. Covalent attachment of nuclear localization signal (NLS) motifs of the SV40-T-antigen and of the HIV-1 Rev protein (ARM) conferred karyophilic properties upon the S4₁₃. The resulting peptides, which were designated as PV-S4₁₃ and RR-S4₁₃ penetrated into intact HeLa cells and were able to accumulate within the cells' nuclei. In studies with digitonin-permeabilized cells, nuclear uptake of the PV-S4₁₃ and the RR-S4₁₃ peptides showed the same features that characterize active nuclear import. Nuclear import was observed at 37 °C, was ATP-dependent, and was inhibited by the free peptides bearing the SV40 NLS and the Rev and Tat ARMs. Microinjected S4₁₃ remained in the cytoplasm while microinjected RR-S4₁₃ was translocated into the cells' nuclei. The new type of cell-permeable “karyophilic” peptides described here may be of potential application as a lead compound for therapeutic purposes, as a tool to study nucleocytoplasmic shuttling in intact cells, and for the delivery of peptides to the nucleus.

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Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

ISSN (print) / ISBN 0006-2960

e-ISSN 1520-4995

Journal Biochemistry

Quellenangaben Volume: 41, Issue: 29, Pages: 9208-9214

Publisher American Chemical Society (ACS)

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Institute of Virology (VIRO)