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Kuppner, M.C.* ; Gastpar, R. ; Gelwer, S.* ; Nössner, E. ; Ochmann, O.* ; Scharner, A.* ; Issels, R.D.

The role of heat shock protein (hsp70) in dendritic cell maturation : Hsp70 induced the maturation of immature dendritic cells but reduces DC differentation from monocyte precursors.

Eur. J. Immunol. 31, 1602-1609 (2001)
DOI
Open Access Green as soon as Postprint is submitted to ZB.
Members of the heat shock protein (hsp70) family are either constitutively expressed (hsc70) or can be induced by hyperthermic stress (hsp70). Recombinant hsp70 (rhsp70) stimulates cytokine production from monocytes and enhances NK cell proliferation and cytotoxicity. Here we demonstrate that rhsp70 binds to immature dendritic cells (DC) derived from monocyte precursors and induces theirmaturation as evidenced by an increase in CD40, CD86 and CD83 expression. Immature DC stimulated to mature with rhsp70 show an enhanced ability to present tyrosinase peptide to specific CTL. MatureDC did not bind rhsp70, suggesting a down-regulation in the expression of its receptor. When rhsp70 was added to monocyte precursors at the same time as GM-CSF and IL-4 it reduced the differentiation of monocytes into DC as shown by a decrease in the level of CD40, CD83, CD86 and HLA-DR expression and an increase in CD14 expression. The constitutively expressed hsc70 had neither a stimulatoryeffect on the maturation of immature DC nor did it reduce the differentiation of monocytes into DC. These findings demonstrate the specific ability of rhsp70 to induce the maturation of immature DC. Therefore rhsp70 may be useful for its adjuvant like properties in DC based immunotherapy of certain tumors.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Dendritic cell Maturation Heat shock protein Hsp70 Hsc70
ISSN (print) / ISBN 0014-2980
e-ISSN 1521-4141
Journal European Journal of Immunology
Quellenangaben Volume: 31, Issue: 5, Pages: 1602-1609 Article Number: , Supplement: ,
Publisher Wiley
Publishing Place Hoboken
Reviewing status Peer reviewed
Institute(s) CCG Tumor Therapy with Hyperthermia (IMI-KTH)
Institute of Molecular Immunology (IMI)