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Open Access Gold as soon as Publ. Version/Full Text is submitted to ZB.
Evidence for Coordinated Induction and Repression of Ecto-5'-Nucleotidase (CD73) and the A2a Adenosine Receptor in a Human B Cell Line.
Biol. Chem. 384, 483-487 (2003)
In the human B cell line P493-6 two mitogenic signals, the Epstein-Barr virus nuclear antigen 2 (EBNA2) and myc, can be independently regulated by means of an estrogen receptor fusion construct or an inducible expression vector, respectively. Shut off of EBNA2, either in the presence or absence of myc, leads to a significant increase in enzymatic activity and surface expression of ecto-5'nucleotidase (CD73) as well as an increased adenosine receptor response in cyclic AMP formation. Shut off of myc expression has a small additional positive effect on CD73 activity. Among the four different subtypes of adenosine receptors, the A2a receptor exclusively is subject to regulation in this system, which is substantiated by pharmacologic data (specific agonists and inhibitors), as well as on the mRNA level. With upregulated CD73 and A2a, cells also respond to 5'-AMP with increased cyclic AMP formation. Turn on of EBNA2 has the reverse effect of repression of CD73 and A2a expression. The time course of both induction and repression of CD73 and A2a is rather slow.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
A2a mRNA 5'-AMP c-myc Cyclic AMP EBNA2
ISSN (print) / ISBN
1431-6730
e-ISSN
1437-4315
Journal
Biological Chemistry
Quellenangaben
Volume: 384,
Issue: 3,
Pages: 483-487
Publisher
de Gruyter
Reviewing status
Peer reviewed