PuSH - Publication Server of Helmholtz Zentrum München: Experimental three-dimensional fluorescence reconstruction of diffuse media by use of a normalized Born approximation.

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Ntziachristos, V.* ; Weissleder, R.*

Experimental three-dimensional fluorescence reconstruction of diffuse media by use of a normalized Born approximation.

Opt. Lett. 26, 893-895 (2001)

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We present a normalized Born expansion that facilitates fluorescence reconstructions in turbid, tissuelike media. The algorithm can be particularly useful for tissue investigations of fluorochrome distributionin vivo, since it does not require absolute photon-field measurements or measurements before contrast-agent administration. This unique advantage can be achieved only in fluorescence mode. We used this algorithm to three-dimensionally image and quantify an indocyanine fluorochrome phantom, using a novel fluorescence tomographic imager developed for animals.

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